im钱包下载安卓|马血清

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2024-03-08 02:00:51

Horse Serum for Cell Culture | Thermo Fisher Scientific - PH

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Home›Life Sciences›Cell Culture & Transfection›Mammalian Cell Culture›Other Animal Sera›Horse Serum for Cell CultureHorse Serum for Cell CultureSee Navigation‹Other Animal SeraHorse Serum for Cell Culture

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What is horse serum used for?

Gibco horse serum is donor-herd collected in New Zealand and can be used as an alternative to fetal bovine serum.Horse serum has been cited for use in several specific cell culture applications:Support of in vitro antibody response to SRBC in place of FBS [1]Production of C2 heterokaryons [2]Isolation and culture of primary neural cells [3]Promotion of myogenic differentiation [4]Supplement in mycoplasma broths and agars [5]In addition to horse serum as a lower cost alternative to FBS, Thermo Fisher Scientific also offers normal horse serum that can be used as a blocker solution in immunoassays.Gibco horse serum is available in both heat-inactivated and non-heat-inactivated formats.

Horse serum vs FBS

Horse serum can be used as a viable FBS alternative in some cell culture applications. One key horse serum benefit compared to FBS is its lower cost. However, horse serum composition does differ from FBS composition, with horse serum tending to exhibit lower growth factor and higher immunoglobulin levels than FBS [6]. Therefore, due to differences in FBS and horse serum, your specific experiment and needs should be carefully considered when deciding whether to use horse serum.

Horse serum collection, manufacturing, and testing

Collected from donor herds in New Zealand, each lot of horse serum is tested for its ability to support the growth of Sp2/O-Ag14 (murine myeloma) cells in control medium containing the test lot of serum. Results are compared to those obtained using control growth medium with a previously characterized reference serum. In addition, Gibco horse serum is EIA (equine infectious anemia) tested.

Order horse serum

16050130, 16050122, 16050114, 26050070, 26050088

Publications

Levy EM. (1980) The ability of horse serum to support an in vitro antibody response. J Immunol Methods 36(2):181–183. Production of Heterokaryons. Stanford University Protocols.Patel MR, Weaver AM. (2021) Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling. Cell Rep 34(10):108829. Sosa P, Alcalde-Estévez E, Asenjo-Bueno A, et al. (2021) Aging-related hyperphosphatemia impairs myogenic differentiation and enhances fibrosis in skeletal muscle. J Cachexia Sarcopenia Muscle 12(5):1266–1279. Ligasová A, Vydržalová M, Buriánová R, et al. (2019) Koberna K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 8(12):1510. Subbiahanadar Chelladurai K, Selvan Christyraj JD, Rajagopalan K, et al. (2021) Alternative to FBS in animal cell culture - An overview and future perspective. Heliyon 7(8):e07686.

Resources

Fetal Bovine Serum Basics Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.Cell Culture BasicsLearn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.Gibco iMatch Sera Lot Matching Tool Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.Related productsCell culture mediaCell culture plasticsCell culture reagentsCell culture instruments and equipmentTransfection products

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血清

普诺赛®血清来自南美纯正血源，涵盖特级胎牛血清和马血清，多种规格可选，满足不同的实验需求。血清生产过程符合cGMP标准,通过多项质量测试，低内毒素,无细菌、支原体、噬菌体、病毒等污染，无外源添加因子，不含激素等。经过近600种细胞系、500多种原代细胞，十万次以上细胞培养验证，只为您放心使用。普诺赛优质纯正的南美胎牛血清，促进细胞增殖, 可用于培养肿瘤细胞、原代细胞以及部分干细胞。历经多年发展，普诺赛公司一直在持续稳定的为广大药物研发、疫苗研发企业及医院、高校等科研机构工作者供应高品质血清，欢迎咨询选购。

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* 注明: 通过更精细化的生长曲线绘制，使用特定对血清较敏感的细胞系以及部分原代细胞进行测试，用CCK-8检测，绘制一周的生长曲线。可以更明显的看出，我们的血清质量在同类型产品中名列前茅。

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普诺赛®文献数量已超过4000余篇，其中已有大量客户使用我们的血清产品开展实验，并取得优秀成果，在Advanced Science、Redox Biology、Environmental Pollution等高分杂志上多次发表文章。

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血清产品系列

由于血清的特性，为了解决血清也不适宜反复冻融的问题，方便客户使用，我们的血清产品有50ml、100ml、500ml以及50ml*10等不同规格包装。可满足不同的实验需求

血清FAQs

普诺赛特级胎牛血清适合培养哪些细胞？

普诺赛特级胎牛血清具有非常卓越的促细胞生长能力，适合培养肿瘤细胞，原代细胞，部分干细胞和娇贵细胞。

血清如何保存, 可以放多久？

贮藏温度≤-15℃，可长时间保存； 2～8℃存放时，请勿超过一个月。

血清溶解以后，出现沉淀怎么办?

若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600g离心5min，上清液即可加入培养基内一起培养。我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。

细胞培养中“低调的高手”——马血清 - 知乎

细胞培养中“低调的高手”——马血清 - 知乎切换模式写文章登录/注册细胞培养中“低调的高手”——马血清灵儿细胞培养中的马血清说起细胞培养用的血清，大多数人都会想到胎牛血清、新生牛血清，对于马血清却知之甚少。今天就给大家介绍一下血清中“低调的高手”——马血清。一、马血清作用细胞培养级的马血清，经过多次过滤，去除了许多大分子蛋白，但保留了血清中促细胞生长的活性因子，表现出与胎牛血清和牛血清的类似的特性，因而能用于细胞培养。马血清可以刺激成纤维细胞分化，以及细胞外基质 (ECM) 、细胞因子的合成。另外培养神经细胞和心肌细胞、诱导生成树突状细胞等实验均可用马血清替代胎牛血清。因此，马血清通常适用于那些研究有丝分裂后，细胞关于生长因子和激素等问题的实验。或许，我们可以这样说，从现有研究来看，马血清更适合用来研究特定种类细胞的分化。实际实验应用方面：马血清已被用作脑皮质神经元-胶质细胞培养的一种必添加物；大鼠的 PC12 细胞，在培养过程中，也需要在基础培养基中添加马血清作为补充剂。1. 马血清可用于细胞培养和干细胞的诱导分化，例如：（1）RPMI-1640培养液加马血清培养脐带血单个核细胞。（参考文献：李春莉, 王建伟, 姜蓉, et al. 人参总皂苷诱导红系血细胞增殖的信号转导[J]. 中国组织工程研究, 2009(23):4568-4572.）（2）原代培养神经细胞。有人用含10%马血清的DMEM，分离培养BALB/c小鼠原代脑神经细胞。发现和添加2%神经细胞生长剂B27的Neurobasal培养基相比，含10%马血清的DMEM培养基更节省成本，方便廉价。（参考文献：李丹, 吕耀中, 王微,等. 两种培养基分离培养BALB/c小鼠原代神经细胞方法的比较[C]// 中国北方实验动物科技年会. 辽宁省实验动物学会, 2015.）（3）诱导脂肪干细胞向成肌细胞分化（文献来源：刘杰,傅强.马血清在脂肪干细胞成肌诱导中的作用[J].中国组织工程研究, 15(14)）（4）培养骨髓基质细胞。与胎牛血清相比，马血清对小鼠骨髓基质细胞的促增殖作用更好。（文献来源：王改琴. 当归多糖对小鼠骨髓基质细胞黏附分子表达及其黏附功能的影响[D]. 学位论文.重庆医科大学, 2008.）（5）添加20%马血清的L-DMEM培养液可诱导人骨髓间充质干细胞分化为脂肪细胞。（文献来源：赵文静，陈亚洁，刘薇.人骨髓间充质干细胞分离培养及生物学特性研究.中国现代医学杂志.2006.16(18):2744-2747）2. 马血清添加到微生物培养基中，可用于培养微生物，如：（1）马血清添加到培养基中用于支原体的培养。在《兽药典》中，含马血清的支原体培养基被用于兽用疫苗的支原体检查。用KM2培养基(含马血清)还可培养猪肺炎支原体。（文献来源：胡茂志焦新安,等.猪肺炎霉形体单克隆抗体的制备与鉴定.《中国兽医科技》 2004年12期）（2）马血清加入到固体和液体培养基中，可用于培养幽门螺杆菌。并且，和羊血清和牛血清比较，添加马血清的培养基更易于分装和保存。（文献来源：刘琳娜, 丁士刚, 张静,等. 几种培养幽门螺杆菌方法的比较[J]. 胃肠病学, 2012, 017(004):240-241.）3. 马血清及激素可共同诱导建立家兔股骨头缺血坏死的模型。（文献来源：洪嵩, 张天宏. 非创伤性股骨头坏死模型的血液流变学及脂质代谢[C]// 2009第一届贵州骨科论坛.）二、注意事项与胎牛血清一样，马血清在使用过程中也应注意几个原则：1、避免反复冻融；2、三步法解冻，及时分装；3、配制好的完全培养基在4℃储存不要超过7天。还有一个大家比较关心的问题，马血清是否需要灭活？我们都知道，胎牛血清由于特殊的采血时间（5-8月胎龄），牛胚胎体内还没有形成完整的补体系统，因而不需要采用热灭活的手段对补体进行灭活。而马血清，在没有特殊说明的情况下，一般采自成年马匹。作为脊椎动物，成年马也拥有完整的补体系统。因而，如果所进行的实验中，涉及补体相关内容，建议大家在使用前对马血清进行热灭活，也可以直接购买商品化的灭活马血清。一些比较成功的实验案例中，所用到的马血清，也是经过热灭活的。相关实验请参考：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111218/Ausbian马血清为细胞培养级。它来自健康马血液，为封闭系统内无菌采血过滤而成，为淡黄色澄清液体，超低内毒素，无溶血，无异物，无细菌、真菌、支原体、噬菌体等。产品描述1、每个批次均具有完整的检测报告，其中含有下列检测：1）理化检查包括外观、pH值、渗透压、血红蛋白、总蛋白、内毒素、蛋白颗粒度等2）无菌检查含细菌真菌检查、支原体检查、噬菌体检查，确保不含这些外源因子。3）病毒检查牛病毒性腹泻病毒（BVDV）为阴性。2、极低内毒素。3、提供试用，养细胞更安心。发布于 2022-05-15 21:58细胞死亡细胞细胞学赞同 3添加评论分享喜欢收藏申请

Horse Serum, heat inactivated, New Zealand origin

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HomeShop All ProductsCell Lines and Blood ProductsSeraHorse and Equine SeraHorse Serum, heat inactivated, New Zealand originGibco™Horse Serum, heat inactivated, New Zealand originCatalog number: 26050070Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceGibco™Horse Serum, heat inactivated, New Zealand originCatalog number: 26050070Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceCatalog Number26050070also known as 26050-070Unit Size100 mLPrice (USD)Contact Us ›Save to listCatalog Number26050088Unit Size500 mLPrice (USD)Contact Us ›Save to listCatalog NumberUnit SizePrice (USD)26050070also known as 26050-070100 mLContact Us ›26050088500 mLContact Us ›Save to listProduct OverviewFiguresVideosRecommendationsRecommendationsDocumentsFAQCitations & ReferencesAdditional InformationRecommendationsGibco™ sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirementsSera Category: SpecialtyOrigin: New Zealand.EIA tested (negative) serum from a donor herd.SpecificationsSpeciesHorse/Equine, HorseCountry of OriginNew ZealandFormLiquidProduct TypeHorse SerumQuantity100 mLSerum TreatmentHeat-inactivatedSterilitySterileProduct LineGibco™Shipping ConditionFrozenContents & StorageStorage conditions: ≤-10°CShipping conditions: FrozenFiguresDocuments & DownloadsCertificatesSearch by lot number or partial lot numberSearchRequest a CertificateFrequently asked questions (FAQs)Citations & ReferencesSearch citations by name, author, journal title or abstract textSearch

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马血清给特别滴细胞特别滴“爱” - 知乎

马血清给特别滴细胞特别滴“爱” - 知乎切换模式写文章登录/注册马血清给特别滴细胞特别滴“爱”absin爱必信-absin，中国人自己的生命科学百宝箱Σ众所周知，胎牛血清是细胞培养界的“香饽饽”，备受胞胞们的“宠爱”，其实还有一个“香煎饼”，大家可知道？对一些“挑食”的细胞，马血清可谓是散发着一股“煎饼的清香”。图1 马血清优级（abs989）一、马血清的第一大功能是“滋养”一些特别的细胞，如免疫细胞类、成纤维细胞类、上皮细胞类、神经细胞类等。需要添加马血清的常见细胞类型如下：1）NK（人外周血自然杀伤细胞）和NK-92（人恶性非霍奇金淋巴瘤患者的自然杀伤细胞）营养体系：74% α-MEM基础培养基（abs9506）＋ 0.2mM Inositol（abs816057）＋ 0.1mM β-mercaptoethanol（abs9592）＋ 0.02mM Folic Acid（abs812845）＋ 100-200U/mL recombinant IL-2（abs04045）+ 12.5 %马血清优级（abs989）+ 12.5% 胎牛血清优级（abs972）+ 1% 双抗（abs9244）2）EL-4（小鼠胸腺淋巴瘤细胞）营养体系：89% DMEM高糖基础培养基（abs9483）+ 10% 马血清优级（abs989）+ 1% 双抗（abs9244）3）L929（小鼠成纤维细胞）和CRFK（猫肾细胞）营养体系：89% MEM基础培养基（abs9503）+ 10% 马血清优级（abs989）+ 1% 双抗（abs9244）4）MCF 10A（人正常乳腺上皮细胞）营养体系：94% DMEM/F12基础培养基（abs9561）+ 5% 马血清（abs989）+ 1% 双抗（abs9244）+ 20ng/ml EGF（abs04176）+ 0.5ug/ml 氢化可的松Hydrocortisone（abs814512）+ 100ng/ml 霍乱霉素choleratoxin+10ug/ml 胰岛素insulin（abs42225219）5）PC-12（Undifferentiated）（大鼠肾上腺嗜铬细胞瘤细胞（未分化））营养体系：79% RPMI1640基础培养基（abs9484）+ 15% 马血清（abs989）+ 5% 胎牛血清优级（abs972）+ 1% 双抗（abs9244）二、马血清第二大功能是诱导分化作用，有文献报道【1】马血清在 5-氮杂胞苷诱导脂肪干细胞成肌细胞实验中发挥重要作用。该研究如下：将第1代长满100 mm培养皿的脂肪干细胞传代至3个6孔板，实验组培养基中加入体积分数为 5% 马血清 +10 μmol/L 5-氮杂胞苷；对照组培养基中单纯添加 10 μmol/L 的 5-氮杂胞苷；空白组为单纯低糖 DMEM 培养基，其余培养、传代及鉴定所需的条件相同。每日光镜观察记录形态，在培养的第 7、14、28、35 天行免疫荧光和流式细胞仪检测肌蛋白表达差异。该文献的结果与结论是免疫荧光检测成肌特异性胞质蛋白表达以及流式细胞检测相应蛋白表达率提示，加入马血清后的实验组脂肪干细胞的成肌速度和诱导成肌所需时间明显比单纯加入 5-氮杂胞苷的对照组更有优势。实验初步表明马血清在促进脂肪干细胞诱导成肌方面起着缩短诱导成肌所需时间的作用。同时，马血清在诱导 C2C12（小鼠成肌细胞）分化为多核肌管方面也发挥重大作用。C2C12 细胞是由 D·Yaffe 和 O·Saxel 建立的小鼠成肌细胞株的亚克隆；该细胞分化很快，形成可伸缩的肌管并生成特征性的肌蛋白。将状态良好的骨骼肌细胞以 0.9×105 接种在96孔板上（每孔100ul），置于培养箱中培养 24h 后，待细胞融合到 70-80% 后，更换成的含有 2% 马血清的完全培养基中，进行诱导分化。（如图2所示）图3 C2C12（小鼠成肌细胞）向多核肌管方向分化另外，也有文献报道【2】传代培养人骨髓间充质干细胞（P3）在 20% 马血清的 L-DMEM 培养液中可分化为脂肪细胞。此外， 10% 马血清可代替 FBS 用于支持绵羊红细胞的体外抗体应答；血清 IgG 制备，白蛋白的提取及生物活性研究；免疫组化（IHC）、酶联免疫（ELISA）等免疫实验的封闭剂。参考文献：【1】刘杰,傅强.马血清在脂肪干细胞成肌诱导中的作用[J].中国组织工程研究, 15(14)【2】赵文静,陈亚洁,刘薇.人骨髓间充质干细胞分离培养及生物学特性研究.中国现代医学杂志.2006.16(18):2744-2747本期讲解就到这里了，如有细胞培养问题，欢迎微信公众号后台留言~注：图源网络和客户，仅供参考学习用表细胞培养类产品推荐（点击查看更多产品信息）货号品名规格abs981胎牛血清（特级）500mlabs972胎牛血清（优级）500mlabs974胎牛血清(标准级）500mlabs9483高糖DMEM培养基500mlabs9484RPMI-1640培养基500mlabs9505MEM培养基500ml发布于 2023-05-05 10:59・IP 属地上海细胞培养NK小鼠细胞赞同 1添加评论分享喜欢收藏申请

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Home›Life Sciences›Cell Culture & Transfection›Mammalian Cell Culture›Fetal Bovine Serum (FBS)Fetal Bovine Serum (FBS) for Cell CultureContact us for the specialist support Fetal bovine serum (FBS) offers essential nutrients and growth factors for the maintenance and growth of cultured cells. Cell-based applications are an integral part of basic research, biochemical studies, vaccine development, and drug discovery to name a few. FBS is the most used serum in cell-based applications as it provides the most robust culture system for the widest range of cell types.Since 1962, Gibco FBS products have been a global market leader. Through Thermo Fisher Scientific’s unwavering commitment to quality, scientists using Gibco FBS have been provided with consistent reliability, service, value, and innovation. Discover the performance differences that Gibco Fetal Bovine Serum can deliver for your research and production.

Performance based on science, not geography

Specifications, not origin, help drive consistent performance. We commissioned an independent research group to survey more than 500 researchers around the globe. This is what we learned:Only 7% of FBS users believe that country of origin is the most important indicator of FBS quality.Almost 80% said they would purchase from categories that were clearly delineated by quality indicators (i.e., endotoxin, hemoglobin, growth performance, virus testing, etc.) that matter to their research.Based on these findings, we transformed our Gibco FBS portfolio into categories focused on quality indicators rather than origin. Now you can easily select the best sera for your needs based on how they perform, not where they came from. This also enables us to minimize supply disruption since there is less reliance on origin.The benefits of our performance-based categories Sustainable supply—more supply through origin flexibility assists in minimizing supply disruption Performance consistency—more supply means more customer choice and more lots with the specifications they desire Product continuity—remove the hassle of updating protocols, which can be based on specifications, the true performance driver, and not part numbers

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Our highest quality FBS for use with the most sensitive cells; up to 70 quality specification tests, including our lowest levels of endotoxin and hemoglobin release specificationsOrder Premium Plus FBSAU originOrder Premium Plus FBSUS originOrder Premium Plus FBSNZ originOrder HI Premium Plus FBSAU origin

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Quality profileValue FBSPremium FBSPremium Plus FBSEndotoxin ≤20 EU/mL≤10 EU/mL≤5 EU/mLHaemoglobin/hemoglobin ≤25 mg/dL≤25 mg/dL≤20 mg/dLSterility: bacterial and fungal testing✓✓✓Osmolality✓✓✓pH✓✓✓Mycoplasma: supplemental testing (H-stain)✓✓✓Mycoplasma✓✓✓Country of origin✓✓✓Oritain™ testing (origin confirmation)–✓✓Biochemical and hormonal profileBiochemical: Alkaline phosphatase, ALT, AST, bicarbonate, bilirubin (total), BUN, BUN/creatinine ratio, calcium, chloride, cholesterol, creatinine, GGPT, glucose, HDL, iron, iron saturation, LDH, LDL, phosphorous (inorganic), potassium, sodium, TIBC, triglycerides (TG), uric acid–✓✓Hormonal: Estradiol, insulin, progesterone, testosterone, thyroxine (T4)–✓✓Virus testingSerology: BVDV serum neutralization titer (Ab)–✓✓Serology: Anti-BVDV antibody test (EMA lots only)–✓✓Virus (9CFR): Bluetongue Virus, Bovine Adenovirus, Bovine Parvovirus, Bovine Viral Diarrhoea Virus, Bovine Respiratory Syncytial Virus (BRSV), Rabies Virus, Reo Virus Cytopathogenic Agents - Including Bovine Herpesvirus 1 (BHV-1/IBR) Haemadsorbing Agents - Including Bovine Parainfluenza Virus 3 (PI-3)✓✓✓Virus (EMA): Bluetongue Virus, Bovine Adenovirus, Bovine Parvovirus, Bovine Viral Diarrhoea Virus, Bovine Respiratory Syncytial Virus (BRSV), Rabies Virus, Reo Virus Cytopathogenic Agents- Including Bovine Herpesvirus 1 (BHV-1/IBR) Haemadsorbing Agents- Including Bovine Parainfluenza Virus 3 (PI-3)–✓✓USDA safety testing: Bluetongue virus (Mexican and Australian origin)✓ (Mexican only) ✓✓ (Australian only) USDA safety testing: Akabane virus (Australian only)–✓✓ (Australian only)Protein electrophoresisIdentification:Electrophoretic profile✓✓✓Identification: Bovine gamma globulin (≤500 mg/L) ✓✓✓Protein: Albumin, alpha globulin, beta globulin, total protein✓✓✓Performance testingRelative growth promotion (RGP), relative cloning efficiency (RCE), relative plating efficiency (RPE)✓✓✓DocumentationCertificate of Suitability (TSE CEP)–✓✓Important to note that our FBS is sourced from BSE-negligible countriesGibco Fetal Bovine Serum cross-reference toolThe Gibco Fetal Bovine Serum cross reference tool can be used to find a direct replacement for other serum suppliers. Find your replacement

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Video: What should you consider when selecting FBS for cell culture?

Learn what scientists consider some of the most important factors in selecting fetal bovine serum for mammalian cell culture.Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications.Explore FBS productsContact us for the specialist supportComing soon Gibco iMATCH Sera Lot Matching Tool

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Customer story: using Gibco FBS for cell culture

Learn how Fanny at Institut Curie in Paris uses FBS to culture cells, supporting studies of cell mechanisms and behaviors.

Why Gibco FBS is recommended by most scientists worldwide

Since its launch over 60 years ago, Gibco FBS has been a part of historical scientific breakthroughs, including cell line development, cloning, and gene editing. Gibco sera are the most cited in global scientific journals and are used and trusted by many top pharma companies. Furthermore, Gibco FBS is origin guaranteed through fingerprinting technology and is ISIA traceability certified.Learn more about FBS fingerprinting and ISIA traceability certification

Extraordinary measures in Gibco FBS manufacturing

Extraordinary measures are taken during the manufacturing and production of Gibco FBS, with careful consideration taken in each step of the journey from farm to bench.Explore the incredible manufacturing journey of Gibco FBS

Other animal sera

Did you know there are lower-cost alternatives to fetal bovine serum? These products include bovine serum, horse serum, newborn calf serum, goat serum, rabbit serum, porcine serum, and chicken serum.Explore other animal sera

Sera for bioprocessing

Gibco sera for bioprocessing can support your cell culture needs as you scale up production. As one of the largest serum suppliers from BSE-negligible risk regions, Gibco serum can help meet biopharmaceutical manufacturing requirements.Learn more about sera for bioprocessing

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Understand the basics of fetal bovine serum

Discover the important factors to consider in FBS selection and learn how scientists are utilizing the benefits of FBS in their research.Learn more

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Have questions about using FBS in your cell culture experiments? Find the answers you need in this extensive FAQ database.Access FBS FAQs Fewer resources | Less wasteWhen minimizing waste in your lab, every little bit helps. The 1 L Gibco bottle uses 38% less source material (32% less for the 0.5 L bottle) than comparable media bottles on the market today. This helps reduce the consumption of non-renewable resources, helping shrink your footprint. Download the Green Fact SheetLearn more about sustainable solutions

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浅谈「马血清」在细胞生物学中的作用 - 知乎

浅谈「马血清」在细胞生物学中的作用 - 知乎切换模式写文章登录/注册浅谈「马血清」在细胞生物学中的作用爱生物的小虫子深耕在生物圈内的科研狗细胞培养用Ausbian马血清马血清经过多次过滤，祛除了许多大分子蛋白，但保留了血清中促细胞生长的活性因子，因而能用于细胞培养。相关科学研究1、马血清可用于细胞培养或诱导树突状细胞，且细胞生长、增殖状况良好。有研究人员分别用胎牛血清和马血清培养马单核细胞来产生树突状细胞（eqMoDC），结果发现，马血清马血清生成的eqMoDC与FBS生成的eqMoDC没有区别。然而，与马血清补充培养基一起孵育的eqMoDC在从单核细胞分化过程中表现出更具特征性的树突状细胞形态。在用马血清培养的eqMoDC中也观察到细胞活力的显著增加。此外，发现在马血清存在下产生的eqMoDC在功能性T淋巴细胞启动能力方面优越，并且引发的非特异性增殖显著减少。参考文献：李春莉, 王建伟, 姜蓉, et al. 人参总皂苷诱导红系血细胞增殖的信号转导[J]. 中国组织工程研究, 2009(23):4568-4572.Ziegler A, Everett H, Hamza E, Garbani M, Gerber V, Marti E, Steinbach F. Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum. BMC Vet Res. 2016 Nov 15;12(1):254. doi: 10.1186/s12917-016-0880-8. PMID: 27846835; PMCID: PMC5111218.2、马血清可用于原代培养神经细胞。有人用含10%马血清的DMEM，分离培养BALB/c小鼠原代脑神经细胞。发现和添加2%神经细胞生长剂B27的Neurobasal培养基相比，含10%马血清的DMEM培养基更节省成本，方便廉价。参考文献李丹, 吕耀中, 王微,等. 两种培养基分离培养BALB/c小鼠原代神经细胞方法的比较[C]// 中国北方实验动物科技年会. 辽宁省实验动物学会, 2015.3、特定脂肪酸产物浓度的马血清，对造血细胞有支持作用研究表明，磷脂酶A2特定脂肪酸产物的浓度马血清，支持多能小鼠造血祖细胞FDCP-Mix细胞自我更新的能力。参考文献Ditz T, Schnapka-Hille L, Noack N, Dorow J, Ceglarek U, Niederwieser D, Schiller J, Fuchs B, Cross M. Phospholipase A2 products predict the hematopoietic support capacity of horse serum. Differentiation. 2019 Jan-Feb;105:27-32. doi: 10.1016/j.diff.2018.12.002. Epub 2018 Dec 6. PMID: 30554008.4、马血清可增强猪单性生殖胚胎植入前，胚层的发育。有研究通过对比不同血清和血清样物质，对猪单性生殖胚胎植入前发育的影响，寻找适合其发育的培养基。对单性生殖胚胎进行猪滤泡液（PFF）、胎牛血清（FBS）、马血清（HS）或猪血清白蛋白（PSA）处理，培养2天后。通过囊胚形成和孵化，发现马血清是增强胚层发育的最有效蛋白质来源。接下来，使用三种不同浓度的HS（10%，20%和30%）来确定改善猪单性生殖胚胎发育所需的适宜HS浓度。所有HS浓度均增加了囊胚细胞数量，降低了囊胚凋亡细胞的发生率，其中20%效果更显著。参考文献Hwang IS, Kim SW, Byun SJ, Kim KW, Chung HK, Park JJ, Gobianand K, Park JK, Yoo JG. The effect of horse serum on in vitro development of porcine parthenogenetic embryos. Reprod Biol. 2012 Mar;12(1):25-39. doi: 10.1016/s1642-431x(12)60075-8. PMID: 22472938.5、马血清添加到微生物培养基中，可用于培养微生物，如：（1）马血清添加到培养基中用于支原体的培养。在《兽药典》中，含马血清的支原体培养基被用于兽用疫苗的支原体检查。用KM2培养基(含马血清)还可培养猪肺炎支原体。（2）马血清加入到固体和液体培养基中，可用于培养幽门螺杆菌。并且，和羊血清和牛血清比较，添加马血清的培养基更易于分装和保存。参考文献胡茂志焦新安,等.猪肺炎霉形体单克隆抗体的制备与鉴定.《中国兽医科技》 2004年12期刘琳娜, 丁士刚, 张静,等. 几种培养幽门螺杆菌方法的比较[J]. 胃肠病学, 2012, 017(004):240-241.Ausbian马血清产品说明Ausbian马血清为细胞培养级。它来自健康马血液，为封闭系统内无菌采血过滤而成，为淡黄色澄清液体，超低内毒素，无溶血，无异物，无细菌、真菌、支原体、噬菌体等。产品描述中国国内屠场采集，当地政府兽医管理局检查合格。每个批次均具有完整的检测报告，其中含有下列检测检测报告（1）理化检查包括外观、pH值、渗透压、血红蛋白、总蛋白、内毒素、蛋白颗粒度等。（2）无菌检查含细菌真菌检查、支原体检查、噬菌体检查，确保不含这些外源因子。（3）病毒检查牛病毒性腹泻病毒（BVDV）等病毒均为阴性。极低内毒素，含量≤5EU/ml，使细胞更健康。可提供试用，养细胞更放心。发布于 2022-09-25 22:59细胞培养赞同添加评论分享喜欢收藏申请

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im钱包下载安卓|马血清

im钱包下载安卓|马血清

Horse Serum for Cell Culture | Thermo Fisher Scientific - PH

特级胎牛血清,马血清 - 武汉普诺赛生命科技有限公司 - 您身边的细胞专家【官方网站】

细胞培养中“低调的高手”——马血清 - 知乎

Horse Serum, heat inactivated, New Zealand origin

马血清给特别滴细胞特别滴“爱” - 知乎

Fetal Bovine Serum (FBS) | Thermo Fisher Scientific - PH

HyClone 供体马血清_细胞培养工艺_思拓凡官网 | Cytiva

浅谈「马血清」在细胞生物学中的作用 - 知乎

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